Welcome to IVF
Welcome to IVF
Welcome to IVF
-FISH on Follicular Fluid to detect Gonadal Sex Chromosome Mosaicism in women undergoing infertility treatment
Gonadal chromosome mosaicism is difficult to diagnose as a gonadal biopsy is required. This cannot be used as a routine procedure. In infertile females undergoing in vitro fertilization (IVF) follicular fluid is obtained during oocyte pick-up. After collecting the oocytes for IVF, the surrounding follicular fluid is generally discarded. We studied the cumulus cells present in follicular fluid by the fluorescence in situ hybridization (FISH) technique and found that this was a novel way of detecting aneuploidy in the ovarian tissue. We could detect Turner syndrome mosaicism in this tissue in a cytogenetically proven case of Turner syndrome mosaicism undergoing IVF. The percentage of chromosomal mosaicism is known to vary in different tissues. We could demonstrate that follicular fluid cells are an easily available alternate source of cells to detect chromosome mosaicism by FISH, in patients undergoing IVF. The study is ongoing to collect more data on follicular fluid cells and compare it with karyotyping from blood and FISH on buccal smears.