Welcome to IVF
Welcome to IVF
Welcome to IVF
-In Vitro Maturation of Germinal Vesicle Oocytes
In an IVF cycle, about 8.5% of retrieved oocytes are immature, at the germinal vesicle stage. Complete maturation of oocytes is essential for the developmental competence of embryos. Human oocyte maturation is considered as the re-initiation and completion of the first meiotic division from the germinal vesicle stage (prophase I) to metaphase II and the accompanying cytoplasmic maturation, for fertilization and early embryonic development. Immature human oocytes obtained from patients undergoing oocyte retrieval can be matured and fertilized in vitro. In vitro maturation can be used as an alternative treatment option to stimulated IVF in a select group of women requiring assisted conception. Germinal vesicle stage cumulus-oocyte complexes collected from patients undergoing oocyte retrieval are being cultured in maturation medium with supplements and those that reach the M2 stage undergo intra-cytoplasmic sperm injection (ICSI). Clevage-stage embryos are obtained. The overall maturation, fertilization and cleavage rates after ICSI of 150 germinal vesicle stage cumulus-oocyte complexes will be determined.