Welcome to IVF
Welcome to IVF
Welcome to IVF
-Fluorescent in situ hybridization (FISH) in slowly cleaving and arrested embryos.
Reproductive wastage is a natural phenomenon to eliminate defective embryos. This has been confirmed by cytogenetic studies on spontaneous abortions and recently, on arrested embryos.The Fluorescence in situ Hybridization (FISH) technique enables rapid detection of common aneuploidies. A contributory cause of IVF failure is a high number of zygotes with missing or extra chromosomes. Individual blastomeres from slowly cleaving and arrested embryos which are of no use in IVF are biopsied using a non-contact diode laser beam, treated with hypotonic solution and fixed on slides with an effort to remove the cytoplasm and expose the intact nucleus. The nucleii are located under phase contrast and photographed. FISH for common aneuploidies is performed on these single cells. The signals are analysed to detect aneuploid, mosaic and chaotic embryos.