Welcome to IVF
Welcome to IVF
Welcome to IVF
Sperm FISH
Fresh semen sampleRoutine analysis of sperm chromosomes is not feasible in clinical practice. However, interphase FISH on spermatozoa offers an accurate and reliable method of analysis even in the presence of a low sperm count.

The percentage of sperm with hypo/hyperhaploidy (nullisomy/dizomy) is calculated to counsel the couple regarding chances of a normal pregnancy.

Sample : Fresh semen sample.
Outstation samples are accepted after partial processing in any andrology laboratory. The ‘swim-up’ sperm and pellet are to be separated in 2 tubes and incubated in hypotonic KCl (0.075M) for 20 minutes, following which a few drops of Carnoys fixative are added. After centrifugation, fresh fixative is added to the pellets before transportation. If the outstation sample reaches within 24-48 hours, it need not be processed.
Note: All the outstation samples should accompany with a copy of semen analysis report at the time of sample collection which should include name of the patient, age, count, motility and morphology of the semen sample.