Welcome to IVF
Welcome to IVF
Welcome to IVF
Treatment options
Depending on the type of infertility that has been diagnosed, several options can be offered to couples. Treatment modalities could include either of the following procedures and even a combination of procedures:

Intrauterine Insemination (IUI)
ntrauterine Insemination (IUI)It is the direct placement of washed sperm into the uterus as close to the oocyte as possible around ovulation time.







In vitro Fertilization (IVF)
In vitro Fertilization (IVF)In vitro fertilization involves the collection of mature eggs from the woman's body. In this, the oocyte and a calculated number of sperm are incubated together in a petri dish in culture medium allowing fertilization to take place. This is followed by transfer of the fertilized embryos into the uterus.



Intra Cytoplasmic Sperm Injection (ICSI)
Intra Cytoplasmic Sperm Injection (ICSI)Intra Cytoplasmic Sperm Injection (ICSI) is a delicate but effective technique, which involves the injection of a single sperm into each egg using a glass pipette which is many times thinner than human hair. This improves the fertilization rate and offers hope to men with severe male factor infertility. The invention of Intracytoplasmic Sperm Injection (ICSI) has revolutionized the treatment of male infertility by achieving high rates of fertilization in patients who previously would have had little hope of conceiving.

ICSI is ideally suited for cases of very low sperm count or zero count, sperm with little or no motility and many sperm with abnormal shapes. ICSI benefits women who are advanced in age, those with endometriosis, immunological complications and unexplained infertility. If IVF has failed in a previous cycle or it less number of eggs have been fertilized with IVF previously, ICSI becomes a suitable option.

Dr. Firuza Parikh's team pioneered this technique for the first time in South East Asia in 1994.

Laser Assisted Hatching (LAH)
Laser Assisted Hatching (LAH)Prior to implantation, the embryo has to escape out of its protective shell known as the zona pellucida by a process known as hatching. If this process is not completed properly, implantation will fail and pregnancy will not occur. We offer Laser technology for Assisted Hatching (LAH), where a laser beam is focused over the zona pellucida making a small opening, between 30-40 microns to facilitate embryo hatching. This technique is particularly useful in cases where the embryo has a thick zona and also in cases with previous failed cycles.

Our centre utilizes the Diode laser which is the safest laser in ART.

We achieved the first pregnancy in India using this technique combined with blastocyst transfer (LAHBT).

Blastocyst Stage Culture
Blastocyst Stage CultureA blastocyst is an embryo that consists of more than 100 cells. It is at the blastocyst stage of development (5 days after fertilization) that an embryo normally moves out of the fallopian tube and into the uterus. Once in the uterus, the blastocyst starts to attach to the uterine lining by a process known as implantation. Using newly developed culture media, a higher implantation rate has been observed following transfer of blastocysts (60%), compared to day 3 (40%) embryo transfer.

In our lab we use a grading system for the embryos to decide whether to transfer at Day 3, Day 4 or Day 5 stage.

Endoscopic surgery (Laparoscopy & Hysteroscopy)
Laparoscopy is a procedure that allows us to look directly at the uterus, fallopian tubes, ovaries, appendix, and other organs through keyhole surgery. Operative laparoscopy enables the surgeon to correct defects in the genital organs and remove tumors & cysts in the uterus, fallopian tubes and the ovaries without the patient undergoing an open surgery. This technique is known as "minimally invasive surgery" and involves minimal discomfort to the patient. This technique is helpful in the presence of adhesions, endometriosis, pelvic tuberculosis, fibroids, ovarian tumors and cysts and in intestinal conditions such as appendicitis.

Hysteroscopy is a procedure, which allows the doctor to visualize the uterine cavity from within. Operative hysteroscopy enables the surgeon to correct defects within the uterine cavity. This technique is very helpful to treat intrauterine septum, adhesions and myomas with minimal discomfort to the patient.

Our team has one of the largest experiences in the country with operative laparoscopy and hysteroscopy. The head of our Endoscopy Dept, Dr Neeta Warty is considered a pioneer in this field.

PESA/TESA/TESE
We offer surgical sperm retrieval techniques for men who suffer from azoospermia (no sperm in the ejaculate). Percutaneous Epididymal Sperm Aspiration (PESA) / Testicular Sperm Aspiration (TESA) / Testicular Sperm Extraction (TESE), are procedures in which sperm from men with azoospermia can be removed from the epididymis or the testis and used to fertilize eggs by ICSI/IMSI. These techniques are utilized in conditions of obstructive and non-obstructive azoospermia.

Our team has one of the largest series in India using epididymal & testicular sperm.
The first ICSI baby in India using testicular sperm was born at Jaslok in 1996.

Preimplantation Genetic Diagnosis (PGD)
Preimplantation Genetic Diagnosis (PGD)Preimplantation genetic diagnosis (PGD) is an early form of prenatal diagnosis. It allows genetic analysis to be performed on early embryos by NEGATIVE SELECTION of the affected embryos prior to implantation, thereby allowing couples to achieve pregnancies through IVF with reduction or virtual elimination of the risk of certain genetic diseases in their offspring thereby bringing them hope of healthy babies.

This is a technique used to select genetically normal embryos. The procedure involves ICSI. Following this, a microsurgical operation is performed on the eight-cell embryo (cleavage stage-day 3) in order to extract a single blastomere. Using a laser, an opening is made in the zona. A single cell is gently removed using a PGD pipette. At this stage, the cells are totipotent - every cell of the embryo can form all parts of the body. Therefore, the removal of one or two cells does not hinder the development of the baby. The cell is carefully fixed on a microscopic slide and then analyzed by the FISH technique.

Alternatively, the biopsy is performed on a blastocyst (day 5 embryo) to extract 4-5 trophectoderm cells which are herniated from the embryo. These cells form placenta, hence removal of 4-5 cells does not affect the fetal growth.
Preimplantation Genetic Diagnosis (PGD)



The objectives of offering PGD are:

  • It increases the chances of implantation
  •  It reduces the chances of spontaneous abortions and chromosomal abnormalities in babies.



PGD helps to improve pregnancy rates and decrease spontaneous abortion rates in:

  • Older age women
  • Women with previous unsuccessful IVF attempts
  • Women with recurrent spontaneous abortions
  • Women who have had to undergo repeated terminations because of abnormality diagnosed on prenatal diagnosis
  • Known carriers of genetic defects
  • Racial and Ethnic association with specific diseases (B-Thalassemia,Sickle cell anemia)

Our hospital is the first in India to offer PGD for some genetic disorders using the FISH technique.


We delivered the first baby in India following PGD for Robertsonian Translocation in 2010.


We have the first baby in India following PGD for Reciprocal Translocation in 2011.



INTRACYTOPLASMIC MORPHOLOGICALLY SELECTED SPERM INJECTION (IMSI)

This technique has added a new dimension to the procedure of ICSI. Normally the procedure of ICSI is carried out at a magnification that is twenty fold. The IMSI procedure uses a specialized lens, which enlarges the sperm seven thousand times. This makes isolation of normal-looking sperm simple. IMSI is particularly useful when there is severe male factor infertility, with a large proportion of abnormal looking sperm, repeatedly failed IVF procedures and repeated miscarriages.